Coding

Part:BBa_K3515000:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-22)


E coil alpha helix with cysteine to be used with a K coil alpha helix for protein immobilization.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (C31). Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its five-heptad repeat designed by Chao et al. (https://doi.org/10.1016/S0378-4347(98)00172-8).

References

Chao, H., Bautista, D.L., Litowski, J., Irvin, R.T. and Hodges, R.S., 1998. Use of a heterodimeric coiled-coil system for biosensor application and affinity purification. Journal of Chromatography B: Biomedical Sciences and Applications, 715(1), pp.307-329.